The Worth Way follows for much of its route part of the course of a dismantled railway the Three Bridges to Tunbridge Wells Central Line, which closed in 1967, and serves as an important wildlife corridor. Officially designated a Site of Nature Conservation Importance in an Area of Outstanding Natural Beauty, much of the Worth Way forms an important wildlife corridor supporting a wide variety of fauna and flora. The former railway cuttings and embankments have been progressively colonised by trees, notably Silver Birch, Ash, Hazel and Sallow, which have developed into mature woodland. It supports a variety of flora and fauna, including Nuthatch, Chiff Chaff, Kingfishers, roe deer, foxes, bats and common lizards.
It is part of the National Cycle Network.